Influence of postoperative complications on prognosis of gastric cancer-The manifestation of gastric surgeon's skill, responsibility and empathy / 中华胃肠外科杂志

Radical gastrectomy combined with perioperative comprehensive treatment is the main curable strategy for gastric cancer patients, and postoperative complications are the issue that gastric surgeons have to face. Complications not only affect the short-term postoperative recovery, but also facilitate tumor recurrence or metastasis, thus resulting in poor prognosis. Therefore, unifying the diagnostic criteria for postoperative complications, bringing the surgeons' attention to complications, and understanding the potential mechanism of complications undermining long-term survival, will be helpful to the future improvement of the clinical diagnosis and treatment as well as prognosis for gastric cancer patients in China. Meanwhile, surgeons should constantly hone their operative skills, improve their sense of responsibility and empathy, and administer individualized perioperative management based on patients' general conditions, so as to minimize the occurrence of postoperative complications and their influence on prognosis.

14,15-Epoxyeicosatrienoic acid induces the proliferation and anti-apoptosis of human carcinoma cell

Epoxyeicosatrienoic acids [EETs], which are cytochrome P450 epoxygenase metabolites of arachidonic acid, have anti-inflammatory effects, modulate smooth muscle proliferation, and inhibit smooth muscle migration. This study was designed to determine whether exogenous EETs have any effect on the cell proliferation and apoptosis of carcinoma cell as well as the possible signaling pathways of EETs in this regulation. The effects of EETs on the proliferation and anti-apoptosis of human carcinoma cells were measured by MTT assay and flowcytometric analysis, and the regulation of PPARy, epithelial growth factor receptor [EGFR], extracellular signal-regulated kinase [ERK], phosphatidylinositol 3 [PI3]-Kinase/AKT pathways was investigated by reverse transcriptase polymerase chain reaction [RT-PCR] and western blot analysis. Results of this study suggested that 14, 15-EET may activate the expression of PPARy in Tea-8113 cells. 14,15-EET may stimulate cell proliferation, and increase the percentage of cells during S-G2-M phase in Tea-8113 cells significantly. The levels of EGFR, ERK, and PI3 kinase/AKT proteins were significantly induced by treatment of 14, 15-EET and 14,15-EET/ AUDA, but no significant changes were observed by addition of GW9662. These findings suggest that exogenous 14,15-EET has potent inhibitory effect on proliferation, and could induce apoptosis in Tea-8113 cell, and these changes are related to the expression of PPARganna, the activation of EGFR, ERK, and PI3 kinase/AKT proteins

Pharmacokinetics of dl-praeruptorin A after single-dose intravenous administration to rats with liver cirrhosis

As a novel drug in the treatment of cardiac diseases, dl-praeruptorin A [Pd-Ia] is the major active component of traditional herbal medicine Peucedanum praeruptorum Dunn and is metabolized primarily via cytochrome P450 isozymes [CYP] 3A1 and 3A2 in rats. In the present study, the influence of liver cirrhosis on pharmacokinetics of Pd-Ia and hepatic mRNA expression of CYP3A1 and 3A2 in rats with experimental liver cirrhosis [LC rats] were evaluated. Pd-Ia was given intravenously [5 mgúkg-1] to LC rats induced by dimethylnitrosamine and pharmacokinetic variables were measured. Enzyme kinetic metabolism of Pd-Ia in rat hepatic microsomes was also investigated and hepatic mRNA expression of CYP3A1 and 3A2 were measured by real-time PCR. After intravenous administration in LC rats, the area under the plasma concentration-time curve from time zero to infinity [AUC0-infinity] was significantly greater than that in control rats, which might be due to slower rate of the hepatic blood flow and significant slower hepatic intrinsic clearance [CL int] in rats. The decreased metabolic clearance of Pd-Ia in LC rats might be at least partly caused by the decreased levels of CYP3A1 and 3A2 responsible for Pd-Ia metabolism. These findings may provide new insights into the inter- and intra-individual pharmacokinetic variability of Pd-Ia

anti-inflammatory properties of interleukin 18 binding protein in rheumatoid arthritis

Interleukin-18 binding protein [IL-18BP] is functioning as a natural anti-inflammatory and immunosuppressive molecule by neutralizing the effects of IL-18 during inflammation. This study aimed to identify the role of IL-18BPa in the regulation of immune responses associated with the pathogenesis of RA. 65 RA patients, 22 OA patients, and 40 sex and age matched healthy donors were enrolled in this study. Synovial specimens were obtained through synovectomy or arthroscopic procedures. SFMC and PBMC were prepared by using Ficoll-Hypaque separation procedure. Superarray analysis was used to measure the expression profile of immune-related genes in normal PBMC treated with recombinant human IL-18BPa. The mRNA levels of Th1 and Th2 cytokines were measured by Real-time PCR, and the protein levels of IFN-gamma, IL-4 were detected by ELISA. SuperArray analysis of immune related gene expression profile in normal PBMC treated with IL-18BPa indicated decreases in the gene expression of IFN- gamma and its regulatory molecules STAT-1 and STAT-2. This study pointed out that IL-18BPa has additional anti-inflammatory property through downregulating the expression of IFN- gamma and IL-12, at the same time, upregulating the expression of IL-4 and IL-10. Both IFN- gamma and IL-12 could upregulate the mRNA and protein levels of IL-18BPa in both the normal and RA subjects. Our results demonstrated the importance of IL-18BPa as an immune regulatory molecule and as a promising therapy for treating RA

Impact of quality control on radiation doses received by patients undergoing abdomen X-ray examination in ten hospitals

The X-ray machines used for radiodiagnosis should meet certain quality assurance [QA] programmes. These are necessary to have good quality radiographs at reasonably low exposure to patients. Dose reduction methods in abdomen X-ray examination were carried out in 10 hospitals in Tehran. This paper presents the work, which was implemented on 200 patients and evaluated using the entrance skin dose [ESD] in the Anterior-Posterior [AP] abdomen projection measured directly at the center of the X-ray field. In addition, the machine room, and dark room parameters, as well as work practices and repeat rates were studied. The quality control [QC] parameters and the ESD were evaluated utilizing an anthropologic phantom to define the optimal exposure condition at all hospitals before and after QC. Results show that after using the QC parameters and optimization of the exposure condition s, the mean of mAs and ESD can be decrease d by 62% and 65% respectively. The quality of the radiograph s generally increased. The reported method is easily implemented in any clinical situation where optimization of abdomen radiography is necessary

Research on expression of human papillomavirus type 16 and telomerase in oral lesions / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To define a correlation between human papillomavirus (HPV) type 16 and telomerase activity during the carcinogenesis of oral mucosa.</p><p><b>METHODS</b>HPV16 DNA and human telomerase reverse transcriptase (hTRT) mRNA were detected in 82 cases of paraffin embedded tissues including 7 cases of normal oral mucosa, 7 cases of hyperplasia lesions, 30 cases of oral dysplasia lesions and 38 cases of oral squamous cell carcinomas (OSCCs) by PCR and in situ hybridization (ISH) respectively.</p><p><b>RESULTS</b>HPV16DNA was positive in 14.3% (1/7) of normal oral mucosa, 42.9% (3/7) of hyperplasia lesions, 66.6% (20/30) of dysplasia lesions and 92.1% (35/38) of OSCCs. hTRTmRNA was detectable in 30.0% (9/30) of oral dysplasia lesions and 81.6% (31/35) of OSCCs while normal oral mucosa and hyperplasia lesions were negative. Expression of HPV16DNA and hTRTmRNA were co-ordinate in 67.0% (55/82) cases.</p><p><b>CONCLUSIONS</b>HPV16 infection may play an important role in carcinogenesis of oral mucosa by activation of telomerase.</p>